Biochemistry
Xigen International LLC has interests in biochemical research of recently discovered Diabetes Mellitus treatments. Specifically the Glucagon like Peptides. These class of peptide hormones have been identified to induce the "Incretin effect" during specific phases of human metabolism.
Biochemical Manipulation of Human Carbonic Anhydrase II Enzyme
As an introduction to utilization of Pymol Software and other biochemical calculational technology. We looked at the theorized effects on enzymatic properties of HCAII with specific amino acid residue mutations. These specific mutations focused on residues involved in the reaction mechanism or activ…
Gene Cloning for Testing Mutant Human Carbonic Anhydrase II Enzymatic Properties
Purpose We previously used PyMol modeling software to perform mutagenesis on specific amino acid residues within the active site of HCAII. In order to determine the changes in enzymatic properties of HCAII. We used several techniques including Gibson Assembly PCR to clone both the wildtype and mut…
