Seminar Presentation of Holz. 1999 Article about the GLP-1 Intracellular Signaling with Ca2+ and RyanodineR Stores This article published by Holz. et al. in the Journal of Biomolecualar Chemistry. Looked at the effects of GLP-1 on the Ca2+ influx into the cell that results in a cascade of effects that amplify the influx of Ca2+. This influx of Ca2+ into the pancreatic beta cells is widely
Gene Cloning for Testing Mutant Human Carbonic Anhydrase II Enzymatic Properties Purpose We previously used PyMol modeling software to perform mutagenesis on specific amino acid residues within the active site of HCAII. In order to determine the changes in enzymatic properties of HCAII. We used several techniques including Gibson Assembly PCR to clone both the wildtype and mutant HCAII gene into
Biochemistry Featured Biochemical Manipulation of Human Carbonic Anhydrase II Enzyme As an introduction to utilization of Pymol Software and other biochemical calculational technology. We looked at the theorized effects on enzymatic properties of HCAII with specific amino acid residue mutations. These specific mutations focused on residues involved in the reaction mechanism or active site of the HCAII enzyme.
